Evaluación de la microbiota y resistencia a antibióticos en las aguas termales Ojo de Fantasma, en la comunidad Pungal de Puela del cantón Penipe de la provincia de Chimborazo.

Abstract

The present work has the objective of the Evaluation of the Microbiota and the Resistance to Antibiotics of the bacteria isolated from the thermal waters Ojo de Fantasma of the Penipe canton, Province of Chimborazo, the research was developed in the Microbiology Laboratory of the Faculty of Sciences of the Higher Polytechnic School of Chimborazo, performing the quantification of bacteria in three natural pools using the 3M Petrifilm method (E. coli / Coliformes, Staphylococcus, Mesophilic Aerobias, molds and yeasts), incubating at temperatures of 44 ° C and 37 ° C from the clones obtained, four rings were made to obtain 41 pure clones, for further studies of their macroscopic descriptions, Gram stain where 33 clones (80%) Gram (-) Bacilli and eight clones (20%) were obtained ) Cocos Gram (+). The biochemical characterization, bacterial identification: 11 clones Pseudomonas aeruginosa, six Escherichia coli, five Enterobacteria aerogenes, five Aeromonas schubertii, five Aeromonas schubertii, three Pseudomonas stulzeri, three Brevundimonas, three Staphylococcus aeurus, three Micrococcus luteus and two Enterococcus flavescens. The determination of the Resistance to Antibiotics of the isolated clones showed the Gram negative bacteria showed multiple resistance against ampicillin, penicillin, amoxicillin / clavulanic acid and cefalotin were: Escherichia coli, Pseudomonas aeruginosa, while in Gram positive cocci Staphylococcus aureus, were resistant ampicillin and penicillin, thus demonstrating the presence of a pathogenic microbiota of high health risk for users. It is recommended that the community responsible for local tourism take the necessary measures to improve the quality of the thermal waters.