Evaluación del extracto acuoso de Tagetes erecta. en el control de la marchitez bacteriana (Ralstonia solanacearum), presente en clones de eucalipto tropical (Eucalyptus urograndis).

Abstract

The present investigation proposes: to evaluate the effect of aqueous extracts of the dead flower (Tagetes erecta) in the control of the bacterial wilting produced by Ralstonia Salanacearum, present of commercial plantations of Eucalyptus urograndis in the Los Angeles farm, Buena Fe Canton, Los Rios Province, which belongs to the company NOVOPAN DEL ECUADOR S.A; to solve this problem has been raised the possibility of using botanical extracts. Insulation of Ralstonia solanacearum strains was performed to test the efficiency of aqueous extracts: decoction, infusion, cold macerated, acid extraction and 5 % acetic acid (vinegar). The experimental design used was completely random block design (RBD) and it was structured of 28 treatments and 5 repetitions. There were 4 chemical controls (copper hydroxide, metalaxil, pentahydrate copper sulphate and streptomycin (water). It was effected two methods to perform the treatment evaluation. The first was the method of pouring in plate where decoction treatments were tested at 25, 50 and 100%; Infusion at 25, 50 and 100% macerated in water at 25, 50 and 100% copper hydroxide and Metalaxil, with this methodology the evaluation every 24 hours for 5 days. The second method was an antibiogram where solutions containing 5% acetic acid of antagonistic potential were determined and evaluated by measuring the inhibition halo of treatments at 24 hours. Subsequently the phytochemical screening of the Plant. T. erecta plant was performed. At the end of the trial it was determined that the antagonistic capacity of the aqueous extract is zero as it has no control for R. solanacerum.