Estudio comparativo in vivo de la actividad ansiolítica y antidepresiva de un aceite enriquecido en cannabidiol (CBD) frente a medicamentos de uso farmacoterapéutico común en ratones Mus musculus

Abstract

The objective of the research was to evaluate the anxiolytic and antidepressant activity in vivo of an oil enriched with Cannabidiol (CBD) versus drugs for pharmacotherapeutic use: Nervinetas® and Amitriptyline; apply in mice (Mus musculus). In first instance the setting of the specimens was carried out, then, on the generation of anxiety and depressive processes by modifying a number of environmental factors like: nutrition, hydration, manipulation, and the circadian cycle. The CBD oil was then conditioned and control the doses through dilutions for the respective study, to conform each one of the treatments. For the evaluation of anxiolytic and antidepressant activities, 24 male mice weighing approximately 25-30 grams, were administered orally with CBD oil in doses of 0.429 mg/Kg and 0.857 mg/Kg, oleous carrier (zero), Nervinetas® (anxiolytic control), Amitriptyline (antidepressant control). The not conditioned model to measure anxiolytic activity were: Light Field, Dark Field, and a raised cross maze. The not conditioned models to measure antidepressants activities were: Tail hold and forced swimming. The data was collected through observation and recording on 2 parameters: The number of transitions and the time (seconds). Results were analyzed in the IBM SPSS Statistics 25 program; concluding that the dose of CBD oil with the highest activity, both anxiolytic and antidepressant was the 0.429 mg/Kg which help to achieve a significant decrease on the symptoms of pathologies. It is highly recommended to develop a statistical equipment and a software that could be useful on the execution of these tests: Tail hold and forced swimming, to obtain a more precise, exact and reliable data.