Evaluación de la actividad cicatrizante de Kalanchoe pinnata (Dulcamara) en ratones (Mus musculus

Abstract

The main objective of this research study was to evaluate the healing activity of the ethanolic extract of the leaves of Kalanchoe pinnata (dulcamara) through induced lesions in mice (Mus musculus). The collected plant material was selected and a macro and a micromorphological study of the fresh plant was carried out. Then, the plant material (leaves) was dried and crushed. Later the quality control of the raw plant drug was carried out, and the identification of secondary metabolites was done employing phytochemical screening. A maceration was prepared, using 50 g of ground plant in 500 ml of 70% ethanol, the extract obtained was concentrated and lyophilized. As a treatment, the lyophilized extract was administered by preparing a base gel, at different concentrations (25 ppm, 100 ppm, and 300 ppm) of Kalanchoe pinnata. For the evaluation of the healing activity, 30 mice (females) from 2 to 3 months of age were used, weighing approximately 25 - 30 g, divided into 6 groups: Group A (white control - without treatment), Group B (control negative – base gel), Group C (herbal ointment “MEBO” 0.25% β-sitosterol), Group D (25 ppm gel), Group E (100 ppm gel) and Group F (300 ppm gel). The length and width of the wound, the formation, and detachment of the scab were measured and observed daily, and the days it took for the wounds to heal in the experimental models were registered. The data obtained were analyzed using statistical tests such as unifactorial Analysis of Variance (ANOVA) and Tukey's test. It was concluded that the extracted gel at 300 ppm had a better healing effect since the wounds healed in 9 days and showed healing similar to the positive control. It is recommended to carry out studies of the extract in other pharmacological activities due to the number of metabolites identified in the phytochemical screening.