Efecto de cafeína y glutation adicionados al medio de crioconservación sobre los parámetros de calidad seminal y en la tasa de concepción en bovinos lecheros.

Abstract

The objective of this research was to evaluate the antioxidant effect of caffeine and glutathione added to the freezing medium on bovine semen quality parameters. The research was carried out at the CIBRAE research center, which belongs to the company Produbiogensa Ltda. The collection was carried out by an artificial vagina using four stallions of the Holstein, Jersey, Brown Swiss, and Simmental breeds. A pre-dilution was made in a 1:1 ratio, divided into three aliquots for the addition of their respective treatments (T0:0 mg, T1:1.94 mg caffeine, T2:1.53 mg glutathione), subjected to a controlled temperature decrease for four hours until reaching 6 ºC (pre-freezing phase), packed in 0.5 ml straws, subjected to liquid nitrogen vapors for 20 minutes, then immersed in liquid nitrogen to reach -196 °C (freezing phase). Macroscopic evaluations for color were creamy white to milky white and had an average pH of 6.8. A bifactorial Completely Randomized Design was used, determining significant differences, where the Holstein breed showed greater aptitude in the cryopreservation processes with individual motility values of 64.27% and vitality of 65.03%. The addition of 1.54 mg of glutathione in the post-freezing phase showed superiority in sperm quality values concerning individual motility at 62.5%, vitality at 62.79%, membrane integrity at 61.14%, and acrosome integrity at 67.24%, thus demonstrating its antioxidant capacity and its efficient effect during the cryopreservation process.