Análisis antimicrobiano de los aceites de Anethum graveolens L y Mentha pulegium frente a Candida albicans y Enterococcus faecalis

Abstract

The objective of this study was to evaluate the antimicrobial activity of Anethum graveolens L and Mentha pulegium oils against Candida albicans and Enterococcus faecalis. The research done was experimental since there was a manipulation of the concentrations of the oils and had a quantitative approach. To obtain the essential oils, first the plants were collected and conditioned, the extraction was made through hydro distillation, then quality control tests such as density, IR, yield percentage, physicochemical and organoleptic characteristics were performed; to determine the in vitro antimicrobial effect of the oils, it was made through the disc diffusion method; while, for the determination of the MIC and CMB presented by the strains of interest, it was made by the dilution method. The results showed that the antimicrobial and antifungal effects of the essential oils were determined by the disc diffusion method of Mentha pulegium and Anethum graveolens L at a concentration of 5000 ppm presented inhibition halos against the two strains; the MIC of the oils against Candida albicans was 12.5 μL/mL and against Enterococcus faecalis was 50 μL/mL, the CMB of the essential oils against Enterococcus faecalis were 25 μL/mL for both, the minimum fungicidal concentration of the oils against Candida albicans was 25 μL/mL. In conclusion, the essential oils obtained meet the quality parameters and it could be determined that both oils are sensitive against the two strains. It is recommended to perform a phytochemical screening of Mentha pulegium and Anethum graveolens L to know what type of components and metabolites each plant presents; consider evaluating the antimicrobial activity of the oils against other strains of microorganisms to assess their sensitivity.