Estudio de la actividad antibacteriana (in vitro) de tres especies vegetales de la familia asteraceae frente a una cepa odonto patógena

Abstract

The main objective of this research investigation was to evaluated the antibacterial activity against Streptococcus mutans ATCC 25175 of essential oils of Achillea taygeta, Achillea holoseria, Tanacetum vulgare and the possible potentiation of the effect of chlorhexidine in combination with them. The research approach was mixed, involving the collection and interpretation of qualitative and quantitative results. For the antibiogram analysis, the Kirby-Bauer method was used to establish the sensitivity of the microbial agent to the essential oils and the positive control (chlorhexidine). The combination of the oils and chlorhexidine at 0.05%, 0.12% and 0.2% was evaluated together, incubating the samples at 35°C for 20 hours. As part of the results, the essential oils were found to possess antibacterial activity against Streptococcus mutans. In addition, the inhibition halos of the tested combinations showed an average increase of 4 mm compared to the chlorhexidine control halos. In conclusion, the essential oils studied in combination with chlorhexidine can be used in oral formulations to treat gingivitis and periodontitis whose main cause is Streptococcus mutans, as they act as bacteriostatic and bactericides. In addition, the combination of essential oils and antibiotics results in increased antibacterial activity, which can be a source of research to reestablish the use of antibiotics to which bacteria have developed resistance.