Evaluación de la calidad espermática de semen porcino criopreservado en diferentes niveles de coenzima Q10.

Abstract

The present investigation was carried out with the objective of evaluating the sperm quality of cryopreserved porcine semen at different levels of coenzyme Q10 as an antioxidant. Three stallions from El Oro province, located at an altitude of 119 to 2459 m.a.s.l. with a temperature of 15 °C to 26 °C; of an approximate age of 8 to 10 months of age, three semen extractions were performed on each one and they were divided for the 4 treatments with 4 repetitions; the studies were carried out in animal reproduction laboratory of Animal Science Faculty. For the analysis of the data, a completely randomized design with a combinatorial arrangement of factors was used: factor A, levels of coenzyme Q10 with T0 being the control treatment (without coenzyme Q10), T1 a treatment with the inclusion of 0.04 mg/ml of coenzyme Q10, T2 with an inclusion of 0.06 mg/ml of coenzyme Q10 and T3 with 0.08 mg/ml of coenzyme Q10. Factor B corresponds to the breeds German Pietrain - Duroc Magnus commercial line - Pic 337 commercial line. The results indicate highly ignificant statistical differences for the variables progressive individual motility (p<0.0001); semen viability (p<0. 0001) and membrane integrity (p<0.0001) post freezing obtaining the highest values with T3 (0.08 mg/ml of coenzyme Q10 inclusion) while the commercial line Pic 337 and Pietrain were the boars with the best values post freezing.