Implementación de un sistema de limpieza y desinfección en los criaderos de mantenimiento y maternidad del Bioterio de la facultad de Ciencias

Abstract

The purpose of this research is the implementation of a cleaning and disinfection system in the maintenance and maternity hatcheries of the Faculty of Sciences, with the objective of evaluating the existing microbial load in these areas in order to maintain these levels low through a cleaning system and achieve a quality biotherium. Using the scientific and experimental method, a microbiological evaluation of each of the factors that make up both the macroenvironment and the microenvironment before and after cleaning and disinfection was carried out by means of standard techniques for bacterial counting. All samples taken for microbiological analysis were randomly selected. In the macroenvironment (air, walls, floors, shelves, doors, windows), the existence of mesophilic Aerobes, Staphylococcus aureus, Escherichia coli and molds and yeasts was identified. In the analysis of the microenvironment (cages, litter, drinkers, drinker plugs, feeders, water, feed), mesophilic aerobes, total coliforms, fecal coliforms, Escherichia coli and molds and yeasts were found. The critical points found were the bedding material with 325.67 ×107UFC/g for mesophilic aerobes, 172.83×103UFC/g for coliforms and 269.00×105UFC/g; and the floors with 321.00×107UFC/m2 for mesophilic aerobes, 148.70×105UFC/m2 for coliforms and 178.00×105UFC/m2 for molds and yeasts; and after cleaning and disinfection, the levels were maintained within the standard established by the Spanish Pharmacopoeia for sterile environments, thus determining the effectiveness of the processes implemented to adopt them as routine in the aforementioned housing areas for experimental animals. In the analysis of ectoparasites, a prevalence of 20.22% of mites was found in mice and 30.33% in rats. Therefore, it is recommended to follow the instructions of the procedures for a correct cleaning of the biotherium.