Obtención del consorcio bacteriano nativo del sedimento de la laguna San Antonio del cantón Riobamba

Abstract

The native bacterial consortium was obtained from the sediment of the San Antonio lagoon, Riobamba city, Chimborazo province, in order to make bioremediation possible in contaminated sediments. Sediment samples were inoculated, consecutively isolated until pure strains were obtained, macroscopic and microscopic characterization was performed, then bacterial banks were elaborated with the help of equipment such as autoclave, horizontal flow chamber and incubator. In addition, contaminant degradation tests, antagonism tests and finally the massification of the bacterial consortium were carried out. The result was a total of 7 strains, which after being subjected to a mix of metals, reduced the concentration of 40ppm of Barium to 27.58ppm, in the case of Copper from 5ppm to 0.32ppm and from 6ppm of Vanadium to 0, 30ppm being these results favorable, then the biochemical identification of these strains was carried out giving as a result that the strains L3, L4, L5, L7 and L10 are the microorganism Xenorhabdus nematophilys and the strains L9 and L12 are E. coli - inacticve and in the massification the aerobic count was 38*108UFC/mL, being a good concentration. The application of this bacterial consortium will help to minimize contamination by heavy metals such as copper, boron, barium and vanadium. It is recommended that the Municipality of Riobamba carry out the maintenance and cleaning of the water mirror and sediment of the San Antonio lagoon, through the use and application of native bacterial consortia, since it is presumed that there is heavy metal contamination in the lagoon.