Evaluación de la actividad hipoglicemiante in vivo del componente flavónico de Scoparia Dulcis L.

Abstract

Evaluation of hypoglycemic activity in vivo component flavonic of Scoparia dulcis L, made in the Biotery of the School of Biochemistry and Pharmacy, ESPOCH, in order to seek new alternatives for the treatment of diabetes. For investigation was used, the biological material (18 male rats 290-310g), the same as formed 6 groups of 3 rats: T1 (treatment 1 alcoholic subextract administered 40%), T2 (treatment 2 alcoholic subextract administered 60%), T3 (treatment 3 alcoholic subextract administered 80%), C + (positive control, glibenclamide administered 5 mg / kg), C (negative control glucose solution administered 3 g / kg) and B (white or witness, which no solution was administered). Glycemic levels were evaluated at 0, 30, 60 and 120 minutes after administration of all treatments. Once the administration of treatment, after 30 minutes, induces hyperglycemia with glucose load orally in a ratio of 3 g / kg. The levels of glycemic in the blood after the times mentioned above assessed by spectrophotometer with God- PAD method (enzymatic colorimetric test for glucose). The data obtained were statistically analyzed by the ANOVA test, a factor with grouped data and proof of TUKEY SHD 95% confidence interval. Showing a decrease of 30.21% in treatment 3, on the percentage of commercial drug that reduces by 19.69%. Thus clearly shows that treatment 3 at the dose used, shows hypoglycemic activity, even better than the C +, thus concluding the alcoholic subextract of Scoparia dulcis L, has hypoglycemic activity because of the presence of (quercetin) one flavone component. Subsequently acute toxicity, which was made by histopathology of major organs involved in drug activity (kidney, liver, stomach), reveals no toxic damage, so this alcoholic subextract of Scoparia dulcis L turns out to be safe and effective for the treatment of diabetes. It is recommended that this alcoholic subextract of Scoparia dulcis L, in a dosage form, in order that there are treatment options for elderly diabetic patients, thereby improving their quality of life.