Separación, Purificación e Identificación de Metabolitos Secundarios de Extracto Etanolico de Colca (Miconia pseudocentrophora)

Abstract

It has been carried out Separation, puri fication and identification of secondary metabolites from ethanolic extract of Colca (Miconia pseudocentrophora), the sample was collected in the forest of “San Francisco de Guallabamba” Canton Chambo. To obtain the extract it was performe d: cleaning fresh vegetable, fragmentation and blend with ethanol, rest for 72 hours, filtration; the filtrate evaporated under reduced pressure and removes chlorophylls by means of cooling The results are: liquid in appearance, brown, bitter taste of youn g vegetable, sweetener odor, its pH is 3.43, density 0.97g/ml; it contains terpene flavonoids. Fractionation of 10 mL in column of silica gel of Hexane and Hexane fractions: Acetate, terpenes colored slightly yellow solid are obtained giving bands of 418nm and these are possible saponins, which are passed through chloroform: Methanol water (64.30:10), are developed with vanillin, the fractions eluted with ethyl acetate: Methanol and pure Methanol is obtained possible flavonoids, obtaining 4 to 5 bands which are between 418 and 534nm, chromatography has Rf: 0.87, it was passed through ethyl acetate: formic acid: acetic acid: water (100:11:11:26), the color intensifies with ammonia vapors and intense yellow colors are observed when developing with cerium sulfa te. Compounds that possess a single band may be terpenes, and the compounds having from three to five bands may be flavonoids, their structures must be identified by mass spectroscopy. According to the analysis performed it is recommend to use this plant i n the pharmaceutical industry for drug development.