Estudio microbiológico del manantial termal del Balneario Rumiloma de la parroquia Guangopolo perteneciente a la provincia de Pichincha.

Abstract

This research was performed based on hot springs samples obtained from Rumiloma spa, Guangopolo parish, Pichincha province. The microbiological analysis was developed at biochemical and bacteriological analysis laboratory of Science Faculty of Escuela Superior Politécnica de Chimborazo, where aerobic mesophyllic bacteria, total coliforms, fecal coliforms, yeasts and moulds quantifying tests were developed. The microorganisms quantifying methodology was performed by Petrifilm TM plates method, later colonies were isolated by Mueller-Hinton Agar, macroscopic description, Gram staining, biochemical tests and antimicrobial susceptibility testing from each one of isolated colonies were carried out. Eleven bacterial strains were isolated and identified corresponding to the morphological types of Gram negative bacilli (73%), Gram positive bacilli (18%) and Gram coconut positive (9%) indicating that there is the prevalence of Gram negative bacilli in spa, which is characteristic of hot springs. The species found were Xenorhabdus beddingii (37%), (18%) Brevundimonas diminuta bacteria and (9%) of other bacteria such as: Budvicia aquatica, Pseudomonas stutzeri, Bacillus mycoides, Bacillus spp. and Staphylococcus spp. In fact, in hot springs of Rumiloma spa the presence of a diverse bacterial population were found, some of these as part of the native population and others are on the ground and surface water, that is why from these habitats may reach the spring. We recommend that regular studies be done in order to avoid risks to the health of users.