Evaluación de la actividad diurética del jugo de Caña agria (Costus spicatus) en ratas (Rattus norvegicus).

Abstract

The evaluation of the diuretic activity of the juice of sour cane (Costus Spicatus), it was taken in the Phytochemical Laboratories, Vivarium and Instrumental of the Faculty of Sciences of ESPOCH by using the extract of the branches for the diuretic effect in Rattus novergicus with the average weights of 270 g treated in metabolic cages, through the experimental method of Nail and Col. It was made a pre-clinic study, the preliminary phytochemical analysis included: phytochemical screening, chromatographic analysis and total flavonoids. The diuretic effect of the juice of sour cane was evaluated (Costus Spicatus) administering via oro-pharyngeal, using rats in 5 lots for the experimental groups of treatments to 20, 60, and 80 % of juice of sour cane (Costus Spicatus) and the control groups with the Sodium Chloride and Furosemide. The quantification of the total flavonoids by the method AlCl3 gave as a result 0.56 % of flavonoids. The identification of the possible metabolites was made by layer chromatography, with the solvent chloroform – acetone- formic acid (75:16.5:8.5). Being determined the presence of Kaempferol; the diuresis of the white is 2ml, the positive control (furosemide) gives 6 ml, the 20%, 60%, 80% , of the sugar of sour cane (Costus Spicatus) gives 2.03; 2.70; 5ml of urine respectively. It was concluded that with the juice of sour cane, to 80 % presented a similar diuresis to the furosemide corroborating the traditional usage as non-electrolytic diuretic activity is attributed to the same which is present in other parts of the plant.