Extracción, purificación e identificación de metabolitos secundarios de la planta escama de pescado (Drymaria ovata).

Abstract

This search has like the aim to perform THE EXTRACTION, PURIFICATION AND IDENTIFICATION OF <SECONDARY> METABOLITE (<Drymaria ovata>) PLANT, it was carried out in the laboratory of Photochemistry in the Faculty of Science, the botanical identification was carried out in the herbarium of the ESPOCH an ethanol plant extract was performed by maceration and solvent removal to give a liquid to which the physical properties are determined by photochemical reactions coloring groups, a first fractionation with toluene, ethyl acetate and butanol. TCL (Thin-Layer Chromatography) are determined with different solvents like hexane: ethyl acetate 70:30 reveals with Rosenthales, ethyl acetate: formic acid, acetic acid: water 100: 11: 11: 26 and developed with cerium sulfate to flavonoids. The presence of colored spots rounded and determined the presence of compounds there of which were purified by column< chromatography> and thin layer depending on the complexity and the number of spots. Once purified the work of IR are determined. Photochemical characterization led to positive results terpenes (saponins foam test), pyrogallol phenols (blue ferric chloride), flavonoids (with Shinoda red brick) and alkaloids (with Dragendorff precipitated orange ±). <Chromatography>sub-layer present chlorophylls toluene extract in ethyl acetate gave terpenes in greater quantity. <Chromatography> on sub-ethyl acetate extract, it was determined that three compounds purify the butanol sub- purified extract Compound III was isolated. Infrared spectra for the presence of the following possible saponins spheroidal clusters is determined in terpene can be a saponin and a lactone ring cyclic ether, an isonitrile or an aliphatic CH, or an alkene cycle, not by virtue of it can completely isolate the compound. It is suggested the photochemical study with a better quantity of extract of cefadex which separates the molecular weight metabolites.