Uso de distintos sustratos para el desarrollo de la biomasa bacteriana

Abstract

In the Faculty of Animal Science at the Polytechnic School of Chimborazo, the effect of different substrates (molasses and whey) and culture conditions (agitation and static) were evaluated for the development of bacterial biomass of Lactobacillus casei, using ammonium phosphate as nitrogen source and a temperature of experimental of 34 – 36°C. The results were analyzed using mean separation according Friedman (P<0.05). Setting the effect of the substrates and conditions on the specific growth rate, viability, biomass yield and indicator benefit/cost, being the treatment with stirring molasses (MA) which had the highest specific growth rate (0,087h-1) however, the growth in this treatment was affected by limiting substrate (ammonium phosphate), which led to viable cell counts (6,66 Log CFU/ml) and biomass yields (0,11 g.g-1) low, compared to treatment with whey agitation (LA), same that yielded the highest values of viability and biomass yield (7,86 Log CFU/ml y 0,26 g.g-1) with a specific growth rate of 0,052 h-1, it is also the most cost – effective treatment as it recorder a beneficial/cost of 0,063 cents for every dollar invested. The results demonstrate the effectiveness of using whey as a substrate for the production of bacterial biomass and stirring condition as the ideal for the development of microorganisms

Key words: substrate, biomass