Determinación de la actividad fotoprotectora in vitro de los extractos de Oreocallis grandiflora.

Abstract

The goal of this research is to determinate phoprotective in vitro activity of sub-extract ethanol “cucharilla” leaves (Oreocallis grandiflora) to 2000, 200, 20 ppm. We collected all vegetal material, its conditioning and quality control of the raw drug. By using a phytochemical filter of the extracts prepared with solvents of increasing polarity, we could identify qualitatively the presence of phenolic and flavonoid compounds in its chemical composition. We obtained the dry extract that we used in all tests by freeze-drying of freeze liquid extract with no chlorophyll, grass and etanol. A thin layer chromatography (TLC) showed a possible presence of glycosides flavonoid. The sun protection factor (SPF) in vitro, and the totals flavonoid content were determined by spectrophotometric techniques by using Mansur equation and aluminium chloride. We tested the phoprotector activity by using the method of induced cell death which by exposing Escherichia coli bacteria (ATCC 25922) to ultraviolet radiation (UVB), the quantity of surviving colony forming units (UFC) were detected in intervals of 30 minutes, during 3 hours. The contend of totals flavonoid were 53.57 g. equivalent of quercetin per each 100 g. of dry extract. With a value of 13.56 the FPS were 6.21 and 11.82 for the padimate-o and octylmetoxicinnamate. The ethanol sub-extract to 2000 ppm with a high percent of surviving of Escherichia coli showed a better photoprotector effect than the rest of sub-extracts and positive controls of less concentration. We conclude that FPS and the phoprotector effect increases proportionally to the sub-extract concentration. We strongly recommend make clear structurally the responsible compounds of the phoprotector activity to be used as new filters UVB.