Formulación de un protector solar a base de extracto Ñachag (Bidens andicola) y determinación de su calidad.

Abstract

The main purpose of this research was to formulate a sunscreen based on Ñachag extract (Biden andicola) and determine its quality. The quality control of the vegetable drug was carried out. The phytochemical screening demonstrated the presence of phenolic compounds, from which, the flavonoids in the aerial parts of Bidens andicola. The content of total phenols and flavonoids, as well as the capacity to capture antioxidants was determined by spectrophotometric methods. Several formulations of photo protective creams were prepared. The photo protective activity of the Bidens andicola extracts and the photo protective creams were evaluated by determining the sun protection factor by applying the Mansur equation. The quality and safety control of Bidens andicola ethanolic extract, and the photo protective cream were carried out by the tests: HETCAM (Test ofeye irritation by means of the chorioallantoic membrane of chicken egg) and CAMTBS (Test of eye irritation by means of the chorioallantoic membrane of chicken egg with Trypan Blue) respectively. The extract that showed the best characteristics was the one containing the ethanol extract of Bidens andicola at 600ppm, which had an SPF of 23. The content of phenols and total flavonoids was 178 mg GAE / g dry plant and 71.4 mg QE / g dry plant respectively. The effectiveness of the sunscreen to capture antioxidants was verified by obtaining absorptances with the same performance as the standard. The formulation of choice containing olive oil presented an SPF of 23. The HET-CAM and CAM-TBS tests reported that the ethanolic extract and the sunscreen cream are not irritating. The results obtained comply with the COLIPA standard of efficacy of cosmetic products. The photo protective cream is effective and safe at topical level. Microbiological, rheological and stability studies are recommended to guarantee the effectiveness of the cream.