Evaluación de la actividad antiinflamatoria y citotóxica de atranorina y dilactona del ácido pulvínico: compuestos fenólicos bioactivos del líquen Parmelina tiliacea.

Abstract

Parmelina tiliacea is an Ecuadorian lichen that has been little studied in relation to its potential applications. The research was carried out with the objective of establishing the anti-inflammatory and cytotoxic activity of P. tiliacea, by an in vitro cell method based on the use of a soluble tetrazolium salt (WST-1) on isolated neutrophils. Atranorin and pulvinic dilactone were isolated from lichen P. tiliacea by chromatographic methods (flash column chromatography and TLC). The compounds were identified with the help of spectroscopic studies (UV and IR), chromatographic (TLC) and specific identification tests for lichen compounds using chemical reagents established for this type of molecules. The results of cytotoxic activity showed a reduction of cell viability up to 15-20% in the presence of a concentration of 200 μg/mL of these compounds. At the same concentration of 200 μg / mL, the anti-inflammatory activity of atranorin, dilactone of pulvinic acid and acetylsalicylic acid (positive control) were respectively 75.92±0.73%, 73.65±0.44% and 83.77±0.47%, respectively. This study represents the basis for subsequent lichen research in Ecuador.