Evaluación de la Pseudomonas sp. en procesos de biorremediación en una celda de combustible microbiana con metales pesados sintèticos a efectuarse en el Centro de investigación de energías alternativas y ambiente de la ESPOCH, 2016.

Abstract

It was evaluated, the capacity of Pseudomona aeruginosa in biosorption as a process for the bioremediation of metals such as chromium, zinc and lead and in microbial fuel cells for the production of bioelectricity at the Center for Research on Alternative Energies and Environment (CEAA) of the Superior Polytechnic School of Chimborazo. The strain was donated by ICITS-UNACH. The concentrations studied ranged from 0.5 to 2 chromiun; 5 to 20 zinc and 5 to 20 lead (mg/L). It was observed that the pH of the solution and the analysis technique were very important factors in the yield and biosorption capacity of the isolated strain of Pseudomona aeruginosa. The results obtained in the tests show that the bacterium Pseudomona aeruginosa has a higher capacity for biosorption by lead; with an average of 36, 64% at the highest concentration of the metal compared to its similar Cr and Zn respectively; considering that it was a biological technique of bioremediation in synthetic liquid medium. Microbial fuel cells were constructed with strains of bacteria isolated and as substrate was used: lead nitrate, the cell of a total volume of 125 mL consisted of two chambers separated by carbon fiber tissue. As the synthetic lead metal was absorbed by the bacteria inside the Microbial Fuel Cell; it produced bioelectricity; the amount of voltage generated was relatively proportional to the amount of metal supplied and recorded in volts by a DAQ 6900 data acquisition system; directly to a computer. It is recommended the study and experimentation of Microbial Fuel Cells in environmental bioremediation processes that involve a contamination by industries that use heavy metals within their processes; as is the case of mining, metallurgy, electroplating, petrochemical industries, leather industries and / or tanneries.