Análisis de la actividad fungicida y bactericida del extracto de moringa oleifera y petiveria alliacea en cultivos microbianos procedentes de plagas que infectan a mangifera indica

Abstract

The fungicidal and bactericidal activity of Moringa oleifera and Petiveria alliacea leaf extracts against pests affecting Mangifera indica was tested in this research, this was carried out under in vitro conditions. The process for the extract obtention was carried out by means of maceration with ethanolic solvent followed by distillation with rotavapor at low temperatures to better preserve the active principles of the plant whose final product was packaged in amber bottles isolated from light. To identify mango pests, the samples were taken with the Stuart transport medium, followed by the seeding and its replication on nutrient agar; for bacteria, it was carried out using the streak seeding technique and Sabouraud agar for fungi using the puncture seeding technique. Three different concentrations of extract were obtained changing between the weight of solute and the volume of solvent added to agar by means of the disc-plate method, in which the extract was added to a filter paper disc in direct contact with the agar previously inoculated with the strain of interest. The diameter of the inhibition halo, if formed, was analyzed. Favorable results were obtained for the 1:5 concentration, which formed inhibition halos in the two bacterial strains of interest. It is recommended to expand the different extraction methods in order to determine which is the optimal process to obtain plant extracts and promote the use of organic- origin fungicides and bactericides.