Validación del método espectrometría de absorción atómica electrotérmica (3113b) para la determinación de arsénico y plomo en muestras de agua, del laboratorio aqlab de la ciudad del Coca

Abstract

The present research was carried out to identify that the implementation of regulations in laboratories has increased to obtain for obtaining reliable results. However, the lack of validated methods and training of technical staff affects the accreditation and accuracy of results obtained. Therefore, AQLAB laboratory intends to extend its accreditation in heavy metals due to the impact of these metals on health and environment; thus, the aim of this study was to validate the electrothermal atomic adsorption spectrometry method (3113 B) for determination of Arsenic and Lead in water samples. In this laboratory was carried out a procedure based on theoretical research using bibliographic sources and national and international standards. In addition, a technical methodology was implemented that included the performance of five calibration curves for arsenic and lead. These curves allowed determining the linearity of the method, including slope, intercept, errors, and coefficient of determination. Then, six readings per analyst were performed on different samples and the results were recorded in Excel sheets. Using this methodology, statistical calculations were performed to evaluate the limit detection and quantification, repeatability, reproducibility, trueness, and uncertainty of method. In all calculations, values were obtained values that met the acceptance criteria for validation of arsenic and lead in water samples. Thus, it was concluded that the method is reproducible and has an adequate experimental design that complies with the statistical parameters, so the validation statement was accepted. In addition, a strict procedure for handling volumetric materials and reagents should be followed to avoid errors in the resulting data.