Diseño de un sistema biológico con coctel de microorganismos fúngicos para la degradación del gas metano

Abstract

The objective of this research was to design a biological system with fungal microorganisms for methane gas degradation. First, the design of the biofilters was planned and the operating parameters were established, which consisted of methane concentration percentage, residence time, air inlet pressure, air flow rate, filter bed volume, pump flow rate, choice of mineral medium, culture medium and "Saboreaud" agar to inoculate the fungal strains Fusarium Solani and Candida Subhashii Subsequently, the calibration curves of the rotameters, pumps, methane flow meter ( CH4), chromatograph were constructed. Then, the strains Fusarium Solani and Candida Subhashii were inoculated in the "Saboreaud" culture with pH a 5, 6 for 3 days at 100 rpm, the strains were kept active and pure with replicates every 3 weeks, during the inoculation period, arlite and compost were used as filler material, in wich combinations of arlite and Candida Subhashiii, arlite and Fusarium solani, compost and Fusarium solani were made, the pH of the leachate from each of the biofilters was controlled, keeping them at pH 7. For the analysis of the results, the gas chromatography method was used, through the highly sensitive biogas-hydrogen sulfide (H2S) reading method, at the inlet of the system and at the oulet of each of the biofilters. It was concluded that, the production of carbon dioxide (CO2), percentage of CH4 and through a statistical analysis is represented with scatter plots the results of methane decrease was observed according to the number of days of the experimentation. It is recommended to revise the pipes of the whole system to replace the pipes that are deteriorated.