Patogenicidad y susceptibilidad in vitro a fungicidas de Botrytis cinerea Pers. Causante del Moho gris en el cultivo de Fragaria vesca I cv. Albion

Abstract

The present research proposes: to evaluate the pathogenicity and susceptibility in vitro of fungicides of B. cinerea to select products with greater effectiveness in the chemical control of gray mold in straw; obtaining 9 monoporic isolates of Botrytis cinerea, which showed septate and hyaline hyphae, gray colonies, presence of conidiophores arising directly from the mycelium that supported at their ends numerous conidia as cellular. They also produced microsclerotia in microcultures while in petri dishes with PDA culture media they developed large black sclerotia as the cultures aged. All isolates were pathogenic, although the most aggressive isolates were CC-ESPOCH-Bs-6, CC-ESPOCH-Bc-4 and CC-ESPOCH-Bc-7. On the other hand, the 8 active ingredients tested showed inhibitory effect of the mycelial growth of B. cinerea where the concentrations 4x, 2x and 1x were the ones that had the greatest fungicidal effect. The method of immersion without puncture was the one that allowed a better differentiation of the aggressiveness of the isolates, whereas in the method with puncture, the development of the symptoms was very fast, but less resolutive. The fungicides containing fluoxynil + ciprodinil as well as copper iodocitrate were the ones that had the best inhibitory effect on the mycelial growth of B. cinerea showed. With the results obtained in the research, it was demonstrated that the isolates of B. cinerea were the cause of the gray rot of the fruits of fruit cv Albeon and that the 8 active ingredients showed fungicidal activity, although with different inhibitory effects. Based on this experience it will be possible to justify what could be the best rotations of molecules to be used in the field and thus reduce the risk of the emergence of resistant isolates to achieve a better phytosanitary protection of the strawberry crop. Keywords: ANTIFUNGAL, CHEMICAL CONTROL, MYCELIAL GROWTH, PLANT PATHOGEN, FUNGICIDE.