Identificación y aprovechamiento de la cepa Pseudomona spp en una celda de combustible microbiano para la producción de bioelectricidad a desarrollarse en el centro de energías alternativas (ESPOCH), 2016.

Abstract

Identifies the species of strain Pseudomona spp. and the production of bioelectricity was determined that was able to produce in a microbial fuel cell (MFC), the sample was taken from ICITS-UNACH, which was transferred and suitable in the clinical laboratory of the Faculty of Sciences-ESPOCH. For bacterial sowing and replication Mueller and Nutritive agares were used at a temperature of 30ºC. Once the bacteria were replicated, biochemical tests (four media) plus two additional tests were performed at 42 ° C and 23 ° C, after 48 hours the bacterial strain Pseudomona spp. Belongs to the aeruginosa species. The research was done in the Biotechnology laboratory of the Faculty of Sciences - ESPOCH, six MFCs were used; of which, four were treated and the remaining two were the control group, the material in which they were printed was 3mm thick acrylic, containing a volumetric capacity of 125 mL. The MFC was conformed by two chambers an anodic (bacterium) and another cathodic (oxygen) each one possessed an electrode that served like exchanger of electrons. The substrate used was synthetic waste water based on potassium nitrate at a concentration of 30 and 60 ppm of nitrates. It was monitored through a data acquisition system for 15 days, which allowed to measure the output voltage and store in a computer. It was determined that the bacterial strain is capable of producing bioelectricity from a source of nitrogen and the higher the contaminant concentration the higher the bioelectricity production in the tested ranges; at a concentration of 60 ppm at the final average yield of 0.105 V was obtained, at 30 ppm 0.059 V was obtained and a control group was obtained 0.048 V. It is recommended to use MFCs to produce electricity based on chemical compounds and to determine the difference existing whit cells that produce electricity based on biological compounds.