Determinación de parámetros de validación para valoración de vitamina C en jugos cítricos mediante HPLC para la Facultad de Ciencias-ESPOCH.

Abstract

The validation of an analytical method is the key to the development of reliable and reproducible tests within the area of Chemistry, to achieve the validation method IFU # 17a, 2005 was used, being readjusted to work within the laboratory in stable conditions for vitamin C known as L-ascorbic acid. The method developed by means of High Performance Liquid Chromatography (HPLC) has a linear response with a correlation coefficient of r = 0.995 for each of the calibration curves. The detection limit is equal to 1.0221 mg / L and the quantification limit equal to 5 mg / L. The determined working range for the curve is from 5 to 25 ppm for the concentration of Vitamin C (Ascorbic Acid L). The fortification validation range used consists of 15, 20 and 25 ppm for the concentration of Vitamin C in dilution with mobile phase. The coefficient of variation for each fortified was less than 2% for reproducibility and repeatability. The% recovery established from 90 to 110% for each of the fortified levels. The combined and expanded uncertainty of the method for the concentration levels used is less than 8%. The acceptance criterion used for the uncertainty was less than 30%, giving the previous value of uncertainty as acceptable and in this way the validation objective is met.