Evaluación de cuatro sustratos unicelulares para enriquecimiento proteico del bagazo de caña de la Empresa “IANCEM”

Abstract

The objective of the study was to evaluate four unicellular substrates for protein enrichment of sugarcane bagasse of the company "IANCEM" for which the activation of the unicellular substrates Saccharomyces cerevisiae, Corynebacterium glutamate, arthrospira platensis (spirulina) and algae pool was performed, the substrates of bacteria and yeast were subjected to growth for 72 hours and up to 12 days for arthrospira platensis and algae pool, this procedure was carried out using a protocol developed by the Ingenio Azucarero del Norte "IANCEM", with constant agitation and turbidity measurements in the process. The inoculation of the material was accomplished in the four substrates with 500gr of cane bagasse, 500ml of unicellular substrate, 27 g of molasses, 8 g of urea and 2 g of salt, with three replicas for each substrate, and in anaerobic conditions. The average protein content present in sugarcane bagasse at 45 days was evidenced in spirulina 52.86, algae pool 51.66, bacteria 9.23%, and yeast 9.06. For descriptive statistical analysis, the latest version 2022 was used in Microsoft Excel, and data analysis was performed in IBM SPSS Statistics version 25, where ANOVA Analysis of Variance and Tukey test were performed. The most efficient substrate for protein enrichment of sugarcane bagasse to spirulina (arthrospira platensis), and substrates that do not have experimental differentiation are bacteria and yeast. It is recommended to use longer inoculation time to sugarcane bagasse with the unicellular substrates spirulina and algae pool to evaluate if the protein concentration increases.