Estudio Fitoquímico y evaluación de la actividad Antihemorragica IN VITRO e IN VIVO de Brownea ariza Benth

Abstract

Study phytochemical and the anti - blee ding activity in vitro and in vivo Brownea assessment was conducted in order to check its activity to stop and heal blee ding. I t w as proceeded to collect the sample, was conditioned and perf orm the phytochemi cal screening, subsequently be q ualified and quantified the compounds of int erest using UV sp ectroph otometry , IR spe ctrophotomet ric, and chromatography in thin layer TLC. A s s oon as the study was concluded the phy tochemical stud y was to perform tests of cy totoxicity , in vitro and in vivo ; all this by testing: fatality on Artemia salina for cytotoxicity, prothrombin (PT) and t h romboplastin (PTT) for the in vitro tests and bleeding time for in vivo testing. W ith the data obtained was determined that Brownea ariza Benth possesses compounds of type phenolic structure very similar to the tan nic acid and gallic acid, which extracts has the ability to form clots rapidly in form the clot to a concentration of 3,125 ppm. T his checks that Brownea ariza B enth is useful to stop bleeding in a very short ti me, so it is recommended to develop new medicines for topical for wounds of ulcerati ve type as it is the case of diabetic foot in a diabetes mellitus from this plant.