Actividad fotoprotectora In Vitro de extractos de Halenia Weddeliana Gilg.

Abstract

Ecuador, located on the Equator, is exposed to larger doses of ultraviolet (UV) radiation causing different skin diseases such as carcinoma, photo aging, etc. This study aims at improving quality life, so potential photoprotection from different extracts of Halenia weddeliana Gilg (cacho de venado), was evaluated in the present investigation. The study was carried out at the Natural and Clinic Product Laboratory belonging to Escuela Superior Politécnica de Chimborazo. A qualitative phytochemical analysis was done to verify the presence of phenolic compounds. The total phenol and flavonoid contents were found with the Folin-Ciocalteu and aluminum chloride methods, respectively. Later, the sun protection factor (SPF) in vitro was determined using the spectrophotometric method developed by Mansur. Then the photoprotection effect against UVB radiation by cell death induced through exposition to different times using Escherichia coli. The lacton, courmarin, triterpen, steroid, fat, sugar, reducer, saponin, phenol, tannis, quinon, cathequin and flavonoid presence was determined with the qualitative phytochemical analysis. The most characteristic phenolic and flavonoid contents were 15.63 ± 0.14 mg of gallic acid/g and 1.72 ± 0.0009 mg of quercetin respectively for the ethyl acetate extract. The Halenia weddeliana Gilg extracts showed absorption band in the UVB regions with more SPF in vitro 22.55 ± 0.02 related to the ethyl acetate fraction at 25 ppm, which provided better photoprotection – 2 mg/mL to the Escherichia coli irradiated with UVB. In conclusion, it can be used as protoprotection in further pharmaceutical formulations.