Validación del método de limpieza y desinfección para prevenir infecciones Nosocomiales en el Centro Quirúrgico del Hospital del Día de la Fundación Internacional Buen Samaritano Paul Martel.

Abstract

A cleaning and disinfection handbook was developed for the Surgery Center of the International Foundation Buen Samaritano Paul Martel, Riobamba in 2015. It was validated environmentally with microbiological methods such as swab, impregnation and sedimentation. That is why the disposable Petri dishes with TSA for mesophilic aerobic microorganisms and Sabouraud Agar with chloramphenicol were used. For the microbiological sedimentation analysis, the dishes were exposed for 15 minutes taking into account 1 meter high above the floor with an average of 5 Petri dishes for both mesophilic aerobic microorganisms and fungi respectively for each environment of the surgery center. The swab technique was done with a sterile stainless steel template 10 x10 cm and sterile swabs on contamination- sensitive operating room surfaces. The impregnation technique was used to control the staff’s surgical hand hygiene. The whole procedure was repeated 5 times based on the validation plan with ensures the same conditions. Before implementing the cleaning and disinfection handbook for this surgery center, the average of total mesophilic aerobic count 4 colony-forming united (CFU) and the fungus count was 1 CFU. Having applied it, the average of mesophilic aerobic count was 1 CFU and for fungi was 0 CFU. The null hypothesis was rejected to a significance level of a= 0.05, that is, handbook application and elaboration have a positive impact since the microbial contamination level was decreased without exceeding the limits established by international regulations UNE-EN-ISO 14698-1/2:2006. It is recommended that this surgery center carry out the validation process when remodeling and building inside or close the operation room.