Valoración de subgrupos de “A y B” mediante la aplicación de la prueba de tipificación sanguínea en gel y su correlación con Lectinas A1 y H en pacientes del servicio de Medicina Transfusional del Hospital Provincial General Docente de Riobamba.

Abstract

For this investigation was raised to value subgroups "A" and "B" by applying the technique of typing Blood Gel and its correlation with lectins A1 and H in samples of patients served by the transfunctional Service General Teaching Hospital in Riobamba Province Chimborazo in 2015. the scientific method to enunciate laws, manageable principles and demonstrable useful to man, to avoid incompatibilities transfunctional was applied. Materials used in this research; pipettes 50, 25 and 10 microliters, yellow tips, test tubes, gel cards to identify subgroups ABO, A. 47 blood samples were evaluated, 75% corresponds to group A, B and 19% to 6% sat; 38 blood subgroups A1 82%, 10% and 8% A1B A2 was identified. By applying the technique of gel typing blood group antigens A, B and AB identified. Since this technique high sensitivity and specificity; classifying subgroups was achieved using lectins A1, A2 and antigenic correlation, compared to the antigen identification H. mediantes transfunctional reactions crossmatch is prevented using blood group zero, in patients with weak IDs A. This technique used to assess the antigenic load of blood groups and subgroups. It is recommended as transfunctional alternative based on universal blood type, known as zero Transfucionales Services group.