Caracterización anatómica de Ilex guayusa, Dacryodes peruviana, Terminalia amazonia, Triplaris americana, mediante tinciones, procedentes del Parque Botánico Sucua, ubicado en la provincia de Morona Santiago.

Abstract

The present research proposes: anatomically characterize Ilex guayusa, Dacryodes peruviana, Terminalia amazonia, Triplaris americana, by means of stains; from the botanical park Sucúa, located in the province of Morona Santiago; where samples of each species were obtained. The ones that previously had already been identified in the herbarium of the Escuela Superior Politécnica de Chimborazo. For the anatomical study, the wood was divided into two parts macro and microscopic, for the macroscopic characteristics, the woods were previously dried for 25 days, samples were obtained for xylotheque of 2 * 10 15 * cm where we identified: colour, smell, flavour, pores, texture, lustre, veined and grain; for the microscopic characteristics indistinctly, 2 * 2 * 2 cubes were obtained, which were softened in the autoclave at a pressure of 11.16 atm and 121 °C inside sealed glass containers with aluminium foil for a certain time, afterwards 0.3 um cuts of the wooden cubes were made in a microtome identifying the tangential, transverse and radial planes, these samples were placed in petri dishes where they were hydrated in sterile distilled water, then immersed in Safranina dyes at 1.15 %, bright green 2.5%, green malachite 2%, bright blue 2% each for a certain time, in addition it was determined which is the best dye to observe the microscopic characteristics of the wood of each species, immediately proceeded to measure and account for the perimeters of the pores of each species. Keywords: Botanical Park, Anatomical Characterization, Natural Stains.