Desarrollo de un método de campo indicativo de actividad antibiòtica y antimicótica para propòleos y su utilización asociada con Azadirachta indica (NEEM) en la elaboración de una pasta dental.

Abstract

This research aims to establish a methodology for obtaining soft extract of propolis, design and develop field methods to evaluate its antibiotic and antifungal activity and the use of active propolis in the elaboration of toothpaste associated with Azadirachta indica. This study was developed at the request of the Association of Beekeeping production of Chimborazo (ASOPROACH), in view of its need to carry out a study on the possibility of obtaining soft extract of propolis with antibiotic and antifungal activity proven from of the crude propolis of their apiary. The antibiotic and antifungal activity of propolis were evaluated using field methods designed and developed in this work so that they can be used by beekeepers. These methods were tested using conventional, in vitro tests. Additionally, toothpaste was developed with Propolis (CIM) and an infusion of Azadirachta indicates 10%. The CIM of Propolis extract on Saccharomyces cerevisiae was determined by the method of macro dilution in broth, of an initial innocuousness of 3x108 cells/yeast. The field method to evaluate the antifungal activity of propolis use as an indicator the production of CO2 after inoculation of S, cerevisiae in a solution of sucrose to 40%. While the field method to evaluate the antibiotic activity of propolis used raw milk skimmed and as an indicator the clotting time of casein; Verified the effect by mesophiles aerobics count after 24 hours of incubation. The evaluation of a series of concentrations of Propolis defined the CIM at a value of 12.5 mg/ML (p ≤ 0.001). In the field trial of antifungal activity, 12.5 mg/ML of propolis inhibits the development of UPC of yeast without detecting CO2 that would displace the plunger of a syringe. The antibiotic activity of propolis determined with the field method showed a reduction of 75% of the population exposed to propolis at 24 hours of incubation, with apparent coagulation of casein at 84.22 hours.It is concluded that 12.5 mg/ML Propolis presents antifungal activity on S. cerevisiae and antibiotic activity on A. Mesophiles, effect demonstrated by in vitro and field trials. The toothpaste prepared presented a pH of 7.96 and sensory acceptability 60%.