Detección de inmunoglobulinas m (IGM) y g (IGG) para Brucella spp en muestras de sangre de los empleados de la empresa pública mancomunada del trópico húmedo y los técnicos del MAG de Santo Domingo de los Colorados

Abstract

The research study aimed to detect the Immunoglobulins G (IgG) and M (IgM) against Brucella spp, in the employees of the Empresa Pública Mancomunada del Trópico Húmedo (EPMTH) and the technicians of the Ministerio de Agricultura y Ganadería (MAG) of Santo Domingo. For which, the identification of the mention antibodies was carried out through the ELISA test, in samples taken from the administrative and operational staff of the EPMTH and the MAG companies. The EPMTH population studied was 58 employees and for the MAG were 38 employees, which a survey was applied to evaluate the real situation of each one of the workers for both companies and determine the risk factors to which they are exposed. In addition, socializations were carried out in the institutions of study. The analysis of blood samples was performed in the clinical laboratory of the Sciences Faculty of the ESPOCH. With all the data obtained from the samples and surveys, the process of the information in a database in Excel proceeded. Determining that, from the 58 employees of the EPMTH company, thirteen were positive for (IgG) and three positives for (IgM), while the 38 employees of the MAG company, only two tested positive for (IgG) and none for (IgM). According to the data obtained in the survey, it was determined that 67% of the female staff and 77% of the male staff are aware of brucellosis. It was concluded that 44% of the female staff and 19% of the male staff do not use suitable protective clothing, within their work activities. Therefore, it is recommended to the EPMTH and the MAG institutions the implementation of controls and continuous training together with the MSP (Ministry of Public Health), so that the correct use of biosafety implements can be promoted to their employees.