Evaluación de la actividad antiinflamatoria in vivo de un gel a base del extracto hidroalcohólico de las hojas de Sigesbeckia serrata, en ratones (Mus musculus)

Abstract

The aim of the research was to evaluate the in vivo anti-inflammatory activity of a gel made of hydroalcoholic extract of Sigesbeckia serrata leaves. Once the plant was verified and collected, 600g of pre-selected leaves were dried and crushed to carry out the quality control of the crude drug and prepare the extracts for the phytochemical screening, with the concentrated and lyophilized hydroalcoholic fraction, the phenolics and flavonoids quantification was carried out, to prepare the gels at formulations of 5%, 10% and 20%, the combination of the hydroalcoholic extract with various excipients was used, for the evaluation of the anti-inflammatory activity, 36 Mus musculus mice were used, in which a paw edema was induced with 1% carrageenan, once the inflammation had occurred and the respective treatment had been applied, the posterior right paw was measured for a period of 7 hours. In the phytochemical screening, secondary metabolites were found such as: alkaloids, saponins, amino acids, tannins, phenols and flavonoids, the latter two were quantified with 16.28% and 24.42% respectively, the gels applied subcutaneously in the plantar area helped in the anti-inflammatory process by reducing edema according to the period of investigation, through statistical analysis it was found that the different treatments depend on the extract concentration in the gel to produce the anti-inflammatory effect. It is concluded that making the gels was feasible thanks to the fact that the hydroalcoholic part is compatible with the excipients, which allows the extract to be transported, in addition, the 20% gel presents greater activity with respect to the other gels, being a potential alternative for dermal treatment in inflammatory processes. It is recommended to complement the in vivo studies using other methods to determine the different types of pharmacological activities in order to make a phytopharmaceutical drug.