Presentación de semen ovino mediante verificación y congelamiento lento, utilizando diferentes diluyentes comerciales

Abstract

This research was applied at Center for Gene Transfer “REPROGENES” located at 21/2 via a Guano , canton Riobamba,Chimborazo province; the effect of ram semen cryopreservation was evaluated by nitrification and slow freezing, using different comercial diluents over a period 120 days of investigation. At the end of the experiment it was determined that macroscopic characteristics adequate whithin allowable ranges excelling volume presented 0,96+0,04 ml and optimal microscopic parameters, where the mass motility (99,0%) and highlights averaged concentration of 3281,67+39,04x106 Spz/ml, which generated an average of 58 semen doses per ejaculate, also at the end of stabilization period ram semen dilution, significant defference was determined in as for mass motility (98,60 %), individual motility (4,95 ptos), and sperm vitality (97,60 %), when using Triladyl in relation to the other thinners cryoconservation employees also increased profitability was established by using Slow freezing and thinner Triladyl whit 2,017 USD, which means that for every dollar invested in this procedure a net profit of 1,017 is obtained. So we recommend using the stem cryoconservation by slow freezing and thinner Triladyl for semen of ram, since it presented satisfactory results.